Explore the gene expression files of HGECs after P. gingivalis persisters treatment via RNA sequencing

This study investigated how P. gingivalis persisters interacted with human gingival epithelial cells (HGECs) and affected the gene expression of cytokines, and the related signaling pathways, via RNA sequencing. P. gingivalis (ATCC 33277) was treated with metronidazole (100 µg/ml) for 6 hours to generate P. gingivalis persisters (M-PgPs). HGECs with or without IL-1ß pretreatment were stimulated by M-PgPs and P. gingivalis for 24 hours, and those without any treatments served as the blank control (BL). Overall design: HGECs were divided into 6 groups: HGECs without any treatments (BL), M-PgPs treated, P. gingivalis treated, IL-1ß treated (NC), IL-1ß + M-PgPs treated, IL-1ß + P. gingivalis treated. Each group has 3 replications.