Data underlying the publication: Mapping spatial organization of in vitro neuronal networks using high-content imaging

This dataset was generated to study neuronal organization in complete cell culture wells using automated high-content imaging. Primary cortical, hippocampal, and cerebellar cells from mouse brain tissue were plated in 24- or 96-well plates, fixed at 7, 10, or 14 days in vitro (DIV), and stained for neuronal markers. Cortical and hippocampal neurons were labelled with DAPI (nuclei), MAP2 (soma and dendrites), and Ankyrin-G (axon initial segment), while cerebellar Purkinje neurons were identified with Calbindin. Whole wells were imaged via automated confocal microscopy (400 images/well at 10× for 24-well plates; 225 images/well at 20× for 96-well plates). Individual maximum intensity projection images were stitched into full-well reconstructions using an adapted ImageJ plugin.<br>Scripts and code used for generating and analyzing this dataset are available through Zenodo (see at references)