SupTab1 - Spike-ins.xlsx

Supplemental Table 1: Sequencing spike-in sequences, concentrations, and total input.Supplemental Table 2: Alignment matrix detailing non-coding RNA mapping frequencies (percent total reads) for each sample.Supplemental Table 3: Alignment matrix detailing non-coding RNA mapping frequencies (total raw reads) for each sample.Supplemental Table 4: Normalized read counts for annotated non-coding RNA, excluding no-embryo control samples.Supplemental Figure 1: Spike-in sequencing metrics. The number of molecules of spike-in RNA added to a pool of 3 drops (left y-axis) compared to the measured level of spike-in sequences in reads per million (RPM; right y-axis), totaled across all samples. Per-sample measurements are available in Supplementary Table 1.Supplemental Figure 2: Principal component analysis with control samples excluded. ECCM samples cluster by pool size with single drop pools forming a separate cluster, suggesting that our limit of detection is 3 ECCM drops.