Effect of hUCBSC on glioma cells after co-culture (cDNA microarray results of PI3K-AKT pathway).

Human PI3K-Akt PCR arrays (SA Biosciences) were run using cDNA from single and co-cultures of glioma cells with hUCBSC. Real time PCR was carried out and changes in gene expression were illustrated as a fold increase/decrease according to manufacturer's instructions. The cut-off induction determining expression was 2.0 or −2.0 fold changes. Genes that met these criteria were considered to be upregulated or downregulated.