Next generation sequencing based transcriptomic analysis of bile stress response in Lactobacillus salivarius Ren

Lactobacillus salivarius is a member of the indigenous microbiota of the human gastrointestinal tract (GIT). Tolerance to bile stress is crucial for intestinal lactobacilli to survive in the GIT and to exert their beneficial actions. In this work, the Next-Generation Sequencing platform Illumina HiSeq 2000 was used to investigate the global response to bile in L. salivarius Ren, a potential probiotic strain isolated from a healthy centenarian. In the presence of 0.75 g liter-1 oxgall, the transcription of nearly 200 genes was detected to be associated with bile stress, including genes involved in carbohydrate and amino acid metabolism, cell envelope and fatty acid biogenesis, transcription and translation. This study improves our understanding on bile stress response in L. salivarius Ren. Overall design: Whole mRNA profiles of L. salivarius Ren growing in the absence (CK) and presence (BS) of oxgall were generated using Illumina HiSeq 2000 platform and differentially expressed genes were anylyzed.