Kinetic parameters of SULT1A1 wild-type and newly evolved 5C2, 5F8 and D249G mutants with pNP and 3CyC.

aKinetic parameters for sulfate transfer to pNP were determined by fitting the experimental data to the equation described in ref 21 (see also Material and Methods), taking into account the inhibition observed at high pNP concentrations. The parameter ks is the specificity constant at low pNP concentrations and is equivalent to kcat/KM in Michaelis-Menten kinetics. Vp is the rate at the peak and V∞ is the limiting rate when pNP reaches ∞ (Fig. 5).bThe WT parameters for pNP are adapted from Alcombri et al.[22] the kcat/KM is equivalent to ks and Vmax is equivalent to Vp.cThe kcat/KM values for 3CyC are derived from the fit to Michaelis-Menten equation of data obtained with 3CyC for the WT or linear fit to data obtained at low 3CyC concentrations for the different mutants (Fig. 5).