Mutations and indels in C3h-KO and KO-PGIM.

Variants identified from whole-genome sequencing of parental and mutant strains and mapped to the 3D7 reference strain. Following initial quality filtering outlined in the Methods, variants were filtered that did not differ between the 3 strains. The KC5, C3h-KO, and KO-PGIM genotypes are reported as 0 when identical to the reference and 1 when a mutant allele was called. The VAR column reflects the number of parasites that differed from 3D7 at the indicated position. Columns with.DP and.GT suffixes indicate read depth and the primary allele for each parasite, respectively. Blue highlighted rows indicate high-confidence mutations in KO-PGIM relative to C3h-KO; green highlights reflect those in C3h-KO relative to wild-type KC5. (XLSX)