Rmarkdown code and raw data to figures for Expanding the CRISPR toolbox in Culicine mosquitoes: in vitro validation of novel Pol III promoters
收藏DataCite Commons2020-08-26 更新2024-07-27 收录
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https://figshare.com/articles/Rmarkdown_code_and_raw_data_to_figures_for_Expanding_the_CRISPR_toolbox_in_Culicine_mosquitoes_in_vitro_validation_of_novel_Pol_III_promoters/11407752
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Raw data from cell transfection and luciferase assays. Luciferase readings are from Firelfly luciferase normalised against Renilla luciferase control. This is then transformed against a no sgRNA control to produce indirect scales of activity for U6 & 7SK promoters of sgRNA. Data were analysed by GLMMs with a gamma distribution and log link - full analysis code present in Rmarkdown file, along with code to produce the plots for Figure 1 and Figure 2
提供机构:
figshare
创建时间:
2019-12-19



