miRNA and mRNA sequencing in cattle and pig

This study aimed to elucidate the role of ruminant-specific miRNAs in shaping mRNA expression divergence between ruminant and non-ruminant species. Three cattle and three pig blood samples were used for miRNA sequencing. One pooled sample from seven cattle blood samples and three pig blood samples were used for mRNA sequencing. Peripheral whole blood (approximately 2.5 mL) was collected from the jugular vein of cattle and pigs, into PAXgene Blood RNA tubes. TruSeq mRNA and miRNA library was constructed using Illumina System (TruSeq RNA and small RNA sample Preparation Kit). Sequencing was performed on the HiScan SQ system (Illumina) using the TruSeq SBS kit v3 (50 cycels, Illumina).