Mediators secreted 8h or 24h after poly(I:C) stimulation alone or additionally pre-exposed for 24 hours to HDM.

Cell-free supernatants were analyzed for the presence of cytokines and chemokines in relation to stimulation by poly(I:C) with or without HDM pre-exposure. The table shows cytokines that are: HDM-enhanced in response to poly(I:C); HDM-down-regulated in response to poly(I:C) and not affected by HDM pre-exposure. The concentration values are given in pg/mL and standard deviations are in brackets and represent an average of a triplicate experiment. Fold changes (FC) were calculated as a ratio between HDM pre-stimulation followed by poly(I:C) exposure condition to HBSS pre-exposure followed by poly(I:C) stimulation condition. Fold changes and values were considered significant if p<0.05. Statistically significant induction of cytokines production by poly(I:C) compared to non-induced control conditions is marked with (#) and significant effect of HDM pre-exposure to the FC values are marked with (*). B.d – below the detection limit.