Figure A, Construction of the intracellular version of full-length CelA expression vector pDCW170. Figure B, PCR confirmation of expression vector transformation. Figure C, Diagram of the version of full-length extracellular CelA expression vector pDCW173. Figure D, Evidence for extracellular glycosylation of CelA (expanded). Figure E, Purification and verification of extracellular His-tagged CelA. Table A, Primers used in this study.
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