Additional file 10: of HIV-1 and HIV-2 exhibit similar mutation frequencies and spectra in the absence of G-to-A hypermutation

Table S5. Identification and masking of plasmid error hotspots. Plasmid error hotspots (i.e. common sites for background errors due to PCR or sequencing) were identified by examining the distribution of mutation frequencies at every individual position for the HIV-1 and HIV-2 plasmid controls. Plasmid error hotspots were defined as upper outliers within the distribution using the 1.5Â Ă Â IQR rule. Hotspots identified in both HIV-1 and HIV-2 plasmids were masked from all samples prior to mutational analyses, as they would most likely represent background errors in the biological samples.