TAIL-PCR and identification of the T-DNA insertion site in mutant T286 of Verticillium dahliae.
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A) Electrophoresis of TAIL-PCR amplification products obtained from the application of four arbitrary degenerate primers, and three specific primers at each T-DNA border. PCR products from the different combinations (AP1-4) were cloned and sequenced. B) Gene structure of VdCYC8 and the insertional position of T-DNA in T286.
创建时间:
2016-02-22



