Fusarium species used to test specificity of an IGS sequence-based SYBR green or TaqMan qPCR assay designed to detect Fusarium virguliforme.
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Fusarium_species_used_to_test_specificity_of_an_IGS_sequence_based_SYBR_green_or_TaqMan_qPCR_assay_designed_to_detect_Fusarium_virguliforme_/1058141
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Total DNA was extracted from pure cultures of each of these organisms and detection was assessed as cycle threshold (Ct) values generated with qPCR analysis.aSamples of all isolates were 1∶10 dilutions of a 1x DNA extract sample unless otherwise noted.bSamples were processed as described previously but SYBR green Master Mix was used for qPCR detection.
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2015-12-02



