Gene expression altered by Sox2 T258A mutation in mouse embryonic stem cells

mRNA expression profiles of mouse embryonic stem cells harboring Sox2 WT and a Sox2 T258A mutation Overall design: The T258A mutation in which threonine, the 258th residue of Sox2 was mutated to alanine, was introduced into one allele of E14 cells using the CRISPR-Cas9 editing system. Whole transcripts of E14 wild-type cells and Sox2 T258A mutant cells were analyzed by RNA-seq.