Detection of adenoviral DNA in intestinal lymphocytes.

DNAs from intra-epithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) from 24 donors were tested for the presence of adenoviral DNA by nested PCR. The sex (M/F) and age of the donor at the time of biopsy or autopsy is shown. When positive, the sequence of the adenoviral DNA polymerase gene that was amplified was used to assign the species shown (the result of the analysis of whole tissue DNA is also shown). All the identified sequences from any particular individual sample are indicated. The “sequence type” is shown in parentheses after the assigned species in case of sequences isolated more than once and corresponds to the “sequence types” shown in Figure 2.