Dataset for "Precise 3D Tracking of Highly Non-planar Eukaryotic Flagellar Beating Patterns using Digital Holographic Microscopy"

This repository contains all data supporting the publication "Precise 3D Tracking of Highly Non-planar Eukaryotic Flagellar Beating Patterns using Digital Holographic Microscopy" The dataset comprises three core components: Tracking Results High-resolution tracking outputs for two cells, Reclinomonas americana and mouse spermatozoa, captured using the holoV3C algorithm. Each dataset is saved in an Excel file where individual worksheets correspond to sequential video frames, containing (x,y,z) coordinates of tracked flagellar points. Axial Resolution Assessment Derived 3D tracking data for two test objects: 1 μm polystyrene research particles and mouse spermatozoa flagella. Frame-by-frame coordinate sequences alongside consolidated metrics quantifying axial resolution performance across both samples. Processing Time Logs Detailed timing records for each stage of the 3D flagellum tracking pipeline applied to mouse spermatozoa data. Enables assessment of computational performance and identification of processing bottlenecks. For access to raw data and holograms, please contact us. Description of each data file: data S1.Results of 3D tracking of mouse spermatozoa flagellum. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings. The “all_data” worksheet concatenates the full time series of 3D coordinates for the analyzed sequence.data S2.Results of 3D tracking of R. americana flagellum. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings. The “all_data“ worksheet concatenates the full time series of 3D coordinates for the analyzed sequence.data S3.Processing time logs for lateral and axial localization in the mouse spermatozoa flagellum 3D tracking pipeline. For each frame, the elapsed times (s) of each core stage of the tracking pipeline are recorded. The worksheet summarizes average and standard deviation for each stage across the full sequence. The “metadata” worksheet details the experimental settings.data S4.Three-dimensional coordinates of the mouse spermatozoa flagellum used for the axial resolution analysis. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings.data S5.Three-dimensional coordinates of the dispersed polystyrene 1.05-μm research particles used for the axial resolution analysis. Processed (x,y,z) coordinates for each frame of the analyzed sequence are provided in individual worksheets, named by frame number. The “metadata” worksheet details the experimental settings.data S6.Axial resolution metrics. Consolidated performance table summarizing root-mean-square error (RMSE), mean bias error (MBE), and maximum error in axial localization for both test objects - mouse spermatozoa flagellum (data S4) and polystyrene particles (data S5). All metrics are reported in micrometers. The “metadata” worksheet details the experimental settings.