Expression data from tumor samples treated with a tankyrase inhibitor in a mouse xenograft model

Tankyrase enhances beta-catenin signaling via PARsylation and subsequent degradation of Axin, a negative regulator of beta-catenin. Tankyrase inhibitors stabilize Axin and suppress beta-catenin signaling. We developed a novel tankyrase inhibitor, RK-287107. We used microarrays to elucidate the gene expression profile of human colorectal cancer cells treated with RK-287107 in a mouse xenograft model. Human colorectal cancer COLO-320DM cells were subcutaneously injected into NOD-SCID mice. After the tumors were established, mice were treated with RK-287107 (treatment group) or vehicle (control group). RNAs were extracted from tumors of two individual mice for each group and subjected to transcriptome analysis with Affymetrix microarrays.