CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8+ T cell biology, it remains elusive to what extent CRISPR/Cas9 af

NIAID Data Ecosystem50

CRISPR RNA-seq from HepG2 (ENCSR194SPW)

CRISPRi基因编辑

肝癌基因表达

Control CRISPR gRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHcLV07-B) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov

NIAID Data Ecosystem30

Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nuclease

CRISPRi基因编辑

GuideRNA工程

To confirm the tolerance of SpaCas12f1, mismatched assay was performed for NGS.

NIAID Data Ecosystem50

The uncropped raw gel images.xlsx

CRISPRi基因编辑

凝胶电泳验证

Herein, we present the uncropped raw gel images associated with our manuscript titled " Enhancement of CRISPR-Cas12a System through Universal Circular RNA Design ," published in Cell Reports M

Figshare2025-04-29 更新40

hg19 PAM sites

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