Factsheet related paper "protocol for ems mutagenesis application in rice"

The aim of this study is to establish a standard protocol for EMS mutagenesis application in rice. Two studies were conducted to evaluate the effect of four rice seeds presoaking duration (0, 6, 12, and 24 hours), four EMS concentration doses (0.0%, 0.5%, 1.0%, and 2.0%), and four EMS exposure period (6, 12, 24, and 48 hours). Germination rate, plumula and radicle length, seedling survival, shoot length, root length and fresh seedling weight were evaluated.