Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury

In this study, we unveil that transcriptionally distinct oligodendrocyte populations have spatial preference and differential response to spinal cord injury. We also assess by different methods, including single-cell RNA sequencing, that the developmental origin of oligodendrocyte progenitors does not determine their specification into mature olgodendrocyte populations. We pooled the corpus callosum from two adult (P60) Emx1::Cre-Sox10::Cre-GFP-STOP-TdTom mice from which we took two samples (GFP+ and TdTom+ oligodendrocytes) to address the influence of the oligodendrocyte developmental origin in their subpopulation identity in adulthood. The corpus callosum of P60 Emx1::Cre-Sox10::Cre-LoxP-GFP-STOP-TdTom was sectioned at the microtome and microdissected from two mice. The injury site, rostral and caudal wallerian degeneration regions or control spinal cord of three months Sox10::Cre-GFP mice were microdissected from eight injured and five laminectomy mice.