Evx1 is required to regulate appropriate anterior-posterior patterning during murine gastrulation. Mus musculus

We generated loss of function mESC clones by CRISPR-Cas9 to test the function of Evx1 during gastrulation. Evx1Δfs Clone 14 mESCs were differentiated to D4 in serum and compared to unedited mESCs Overall design: Three side by side differentiations (replicates) were performed and sequenced for both the Evx1Δfs Clone 14 mESCs and the WT mESCs. Each side by side differentiation was set up on a different day.