The effects of dissolved and particulate nickel on corals and their microbiome
收藏Research Data Australia2024-12-14 收录
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https://researchdata.edu.au/the-effects-dissolved-corals-microbiome/1377652
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The data describes the microbiome community of the coral Acropora muricata following exposure to dissolved nickel, nickel contaminanted sediments and sediments alone for 7 days under controlled laboratory conditions\nLineage: Amplicon libraries were prepared for sequencing using TruSeq PCR-free kit. The libraries were run as two separate Illumina® Miseq sequencing runs, 2x 300 bp for ITS2 rDNA, 2x 250bp for 16SrDNA and 2x 150bp for 18SrDNA. Sequenced data were processed using a custom pipeline (Greenfield Hybrid Amplicon Pipeline, GHAP) which is based on USEARCH tools (Edgar, 2013). The pipeline is available at https://doi.org/10.4225/08/59f98560eba25.
提供机构：
Commonwealth Scientific and Industrial Research Organisation
