Effect of GDM and insulin on adenosine transport kinetics.

hPMEC were cultured (8 hours) in presence of basal levels of insulin (Basal-insulin), or 1 nmol/L insulin (Insulin) as in Methods. Maximal velocity (Vmax) and apparent Michaelis-Menten constant of saturable transport (Km) were measured for hENT1- or hENT2-mediated adenosine transport. hENT1/2F represents the relative contribution of hENT1 and hENT2 to total adenosine transport and was estimated from Vmax and apparent Km values as described in Methods. *PPP