ChIP-seq analysis of SNAIL transcription factor binding sites in RH30 rhabdomyosarcoma cells

ChIP-seq analysis of SNAIL binding sites in RH30 cells was performed to discover novel SNAIL binding sites in rhabdmyosarcoma cells. ChIP was performed using SimpleChip Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s protocol. For ChIP assays 10 µg of goat antibody against SNAIL (R&D Systems, cat. AB-108-C), . After immunoprecipitation, the DNA was isolated using spin columns from the kit. Subsequently, ChIP-Seq analysis of the immunoprecipitated sample and input sample was performed using the Intelliseq sp. z o. o. (Cracow, Poland) company service in cooperation with Novogene Co., Lt d (Cambridge, UK) using Illumina high-throughput sequencer.