Characterization of expanded viruses.

ND: Not done, envC2V3 PCR was negative.a: Samples for p24 antigen tests(VIDAS HIV p24 II and the domestic HIV-1 p24 assay) and viral load tests(NucliSENS EasyQ HIV-1 v2.0 and COBAS Taqman v 2.0) are culture supernatant of the isolates after expansion.*: NRTI/NNRTI/PI/INI Major drug-resistance mutations. Mutations without “*” are minor resistance mutations, accessory mutations or single nucleotide polymorphisms (SNP).Ref: Reference virus. IIIB_LAI and NL4-3 are reference virus with clear background information and high replication capacity but are not primary isolates.The expanded virus was characterized in terms of infection route, area of origin, CD4+T cells concentration in blood sample, viral RNA concentration (Roche TaqMan v2.0 and BioMerieux NucliSENS EasyQ HIV-1 v2.0), p24 concentration (BioMerieux VIDAS HIV p24 II and One domestic HIV-1 p24 antigen assay), coreceptor usage (Webpssm and Geno 2 Pheno (FPR 2.5%)), the four identical residues at the tip of v3 loop, number of glycosylation sites in v3-loop, and drug-resistance mutations in pol.Characterization of expanded viruses.