scRNA-seq of T cell Independent B cell responses

To fine map the continuum of molecular changes that occur as B cells differentiate to antibody secreting plasma cells in response to the T cell independent antigens LPS and NP-Ficoll we performed scRNA-seq. WT or IRF4-deficient B cells were CTV labeled and adoptively transferred into congenically disparate uMT hosts. One day later, the mice were innoculated with LPS and all transferred cells isolated at 72 hours and profiled on the 10x Genomimcs 5' Library Kit. In addition, we adoptively transferred WT B cells into congenically disparate WT hosts and innoculated the animals with either LPS or NP-Ficoll, and 72 hours later performed scRNA-seq on all responding B cells. Overall design: scRNA-seq on two independent biological replicates of wild-type or IRF4-deficient B cells responding to LPS in uMT mice and wild-type B cells responding to LPS or NP-Ficoll in wild-type hosts.