Intraspecies variability effect in the number of Cas9 and Cas12a candidates targeting multiple or unique AtCBF genes.

Sequence variability in the CBF genes from different Arabidopsis thaliana accessions change the number of candidates that can match multiple targets due to SNPs in the 20 nucleotides of the guide but also SNPs affecting PAM sequence. The use of the standard Col-0 genome reference (TAIR v10) or the corresponding accession genome affects the identification of offtargets thus the correct identification of specific (unique) candidates matching only one CBF gene. The column “exclusive” indicates the number of specific candidates that are only listed when the corresponding reference genome is used.