RNA-seq from monocytes derived dendritic cells (MoDC) samples purified from spleen of C57BL/6 mice to evaluate their gene expression during Plasmodium berguei ANKA (PbA) infection

Dendritic cells have an important role in immune surveillance. After being exposed to microbial components, they migrate to secondary lymphoid organs and activate T lymphocytes. During mouse model malaria, splenic inflammatory monocytes differentiate into monocyte-derived dendritic cells (MO-DCs), which are CD11b+F4/80+CD11c+MHCIIhighDC-SIGNhighLy6c+ and express high levels of CCR5, CXCL9 and CXCL10 (CCR5+CXCL9/10+ MO-DCs). We intend to use these malaria-induced splenic MO-DCs gene expression data to understand more about the migratory route taken by these cells as well the most important genes/pathways involved on this cell differentiation in the spleen, allowing them to migrate to the brain where they develop an important role in cerebral malaria. Overall design: RNA-seq was performed in biological replicates (3 mice per group). Monocytes (CD11b+F4/80+CD11c-DC-Sign-MHC II-from spleen and bone marrow); inflammatory monocytes (CD11b+F4/80+Ly6ChighCD11c-DC-Sign-MHC II- from bone marrow) and MO-DCs (CD11b+F4/80+CD11c+DC-Sign+MHC II+) from splenocytes were purified using a cell sorting ARIA (BD). Monocytes were collected from non-infected mice and MO-DCs from PbA-infected C57BL/6 mice at day 5 post-infection with PbA.