Activated Sludge Containing E. coli Expressing AtzA Synthetic Transcriptome

The objective of this work was to improve our understanding of the quantitative predictive capabilities of metatranscriptomics. To meet this objective, we investigated whether we can predict the activity level of a specific biochemical function based on the abundance of the corresponding gene transcript within measured community metatranscriptomes. In addition, we investigated the lower limit of a microorganism?s abundance that still allows detection of its transcripts within a metatranscriptome and prediction of the activity levels of the enzyme encoded by the transcript. To do this, we amended an undefined microbial community that was derived from activated sludge with varying fractions of an E. coli strain that can catalyze a specific transformation reaction for the herbicide atrazine. This reaction is performed by an enzyme encoded by the AtzA transcript. We observed a linear and proportional relationship between the activity level of the transformation reaction and the abundance of the AtzA transcript down to an E. coli cell density of 0.05% of the population.