Air-liquid interface Caco-2 culture with vasointestinal peptide mimicks gut mucosal barrier function in permeability and bacterial infection

The goal of the project is to investigate the impact of liquid-liquid interface (LLI) and air-liquid interface (ALI) with addition of vasointestinal peptide (VIP) on intestinal barrier properties and mucus production of Caco-2 cell cultures. Caco-2 cells were cultured in transwell plates under LLI and ALI condition and VIP were added to basolateral compartment. LLI-NT, LLI-VIP, ALI-NT and ALI-VIP were collected and analysis using RNAseq.