High-Throughput DNA sequencing reveals the spoilage bacterial and predicted functional diversity in a Brazilian ethanol fermentation plant

Bacteria are spoilage microorganism that may jeopardize the yields of ethanolfermentation due to the potential capacity of competition for nutrients and may provokeyeasts flocculation, a phenomenon undesired in industry. The majority of studies tounravel the biodiversity of bacterial taxa present in ethanol fermentation process werePCR-based techniques which possesses several limitations and lower resolution. Untilthe best of our knowledge there are few papers describing the whole-diversity ofbacterial taxa and their potential sources in ethanol fermentation. For this reason wasemployed the High-throughput DNA sequencing to achieve the bacterial diversityduring an ethanol industrial fermentation based on the massively parallel sequencing of16S rDNA gene and to evaluate the putative potential functions of the microbiota, apredictive analysis as performed. According to previous reports and the findings of thiswork the lactic acid bacteria (LAB) group was the unique group of bacteria accessed bythe HTS in this study and exhibited potential predicted genes for pre-treatment toleranceof sugarcane stem and amino acid metabolism.