Shigella sonnei ΔtolR ΔgalU GMMA-associated proteins identified by proteomics.

GMMA were purified by 2-step TFF from S. sonnei ΔtolR ΔgalU grown in HTMC at 37°C to an OD of 45. GMMA-associated proteins were separated by SDS-PAGE or nano-LC and identified by mass spectrometry. 61 GMMA-associated proteins were identified. The columns show: A) position in list, B) method used to identify each protein (‘1′: identified from total digestion LC/MS-MS, ‘2′: identified from 2D SDS-PAGE PMF, ‘3′ identified from LC/MS-MS and 2D SDS-PAGE PMF, ‘4′ identified from 1D SDS-PAGE PMF), C) annotation, D) gene name, E) accession number. The entries are divided by predicted location. All proteins were analyzed by PSORTb 3.0 and Lipo. If a prediction as lipoprotein was obtained, the protein is listed as lipoprotein irrespective of its PSORTb prediction. Only lipoproteins predicted to be located in the outer membrane have been identified. The other proteins are listed in sections corresponding to their location predicted by PSORTb. For 5 proteins no prediction was obtained by PSORTb or Lipo. These are listed as ‘unknown’.