Supplementary Silver Carp and Gizzard Shad Stable Isotope Data

All specimens were handled and euthanized according to the Murray State University Institutional Animal Care and Use Committee (IACUC) protocol number 2014-008. Fish from all five sites were collected by boat electrofishing with a MLES infinity control box, twin booms, and umbrella array droppers 68.6 m, 10.1 cm bar mesh monofilament gill nets. On June 29th of 2016, Silver Carp and Gizzard Shad were collected from the Ohio River, Clarks River, and downstream of Kentucky Dam on the Tennessee River. These same species were collected from the Illinois River on June 30th of 2016. Lastly, Silver Carp and Gizzard Shad were collected from Kentucky Lake throughout the month of June, 2015. All fish were sacrificed for biological sample collection. We measured total length (mm), and extracted a dorsal muscle tissue sample for stable isotope analysis using a 10 mm stainless steel biopsy punch. After extraction, all dorsal muscle plugs were placed into Whirl-Pak bags then immediately placed on ice and frozen for later processing. White dorsal muscle tissue samples taken for stable isotope analysis from Silver Carp and Gizzard Shad were oven dried at 50˚C for a minimum of 48 hours and ground into a fine powder with a mortar and pestle. After grinding, the samples were placed back into the drying oven for an additional 48 hours to ensure the sample was thoroughly dry. Once dry, the powder was evenly mixed and 0.31- 0.34 milligrams of fish tissue was loaded into Costech pressed 3.5x5 mm tin capsules. Ground samples were analyzed using a Costech Elemental Combustion System 4010 along with a Thermo Delta V Plus Isotope Ratio Mass Spectrometer to obtain isotopic ratios of the samples. The analytical precision of δ13C and δ15N was ±0.06 per mil relative to the standards Vienna Pee Dee Belemnite (VPDB) and air (AIR) for carbon and nitrogen, respectively.