Effect of Large Brown Adipocyte-secreted Proteins on Type 1 Diabetic Mice (NOD) Brown Adipose Tissue

Brown adipose tissue has been recognized as an important endocrine organ for metabolic health. In our study, we found that large proteins secreted by brown adipocyte (CB-100) promote the recovery of euglycemia in type 1 diabetic mice (NOD) when injected at an early stage of the disease. To further elucidate the in vivo effects of CB-100, we analyzed brown adipose tissue from both CB-100 and sham-treated mice. Differential gene expression analysis revealed that BAT from CB-100-treated NOD mice exhibited higher thermogenic activity. This suggests that the recovery of euglycemia in CB-100-treated mice may be attributed to enhanced thermogenic activity of the brown adipose tissue, which also contributes to glucose clearance Overall design: NOD mice with blood glucose range between 190mg/dL and 230mg/dL were injected with CB-100 (>100kDa size fraction of brown adipocyte conditioned buffer) for 7 days (subcutaneous, 1.5mg/kg BW). After 2-8 weeks of post-injection, RNA was isolated from brown adipose tissue.