transcriptional change of the lesion site margins after spinal cord injury in canine

We conduct complete SCI to canine and transplant stem cell into the lesion, in order to observe the transcriptional change of the lesion site margins,we isolate the margin tissue and performing high-throughput sequencing After anesthesia, 2 mm spinal cord segments were taken from each canine, sourced from areas 0-2 mm and 2-4 mm away from the lesion border, and total RNA was extracted. RNA high throughput sequencing was performed by Cloud-Seq Biotech Mid SR mSC: Five days after adding the modified scaffold material to the injury site, isolate tissue samples located 0-2 mm from the injury site Mid SR: Without adding the modified material, isolate tissue samples located 0-2 mm from the injury site after 5 days Acute: Five days after spinal cord injury (SCI), isolate tissue samples located 0-2 mm from the injury site Mid SR 2-4mm: Without adding the modified material, isolate tissue samples located 2-4 mm from the injury site after 5 days Mid nonSR: Sixty-five days after spinal cord injury (SCI), isolate tissue samples located 0-2 mm from the injury site Normal: Isolate 2 mm tissue samples from the same anatomical location in normal spinal cord tissue