Identification of the ubiquitination sites of EV71-VP1
收藏国家人口健康科学数据中心2026-06-06 收录
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EV71-VP1-Flag plasmid was transfected into 293T cells for 36 hours and then treated with MG132 for 12 hours. After drug treatment, cells were collected, cell lysates were incubated with FLAG-Beads overnight and competed with FLAG peptides for 4 hours the next day. Proteins were separated by SDS-PAGE and analyzed by mass spectrometry after Coomassie brilliant blue staining.
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北京蛋白质组研究中心创建时间：
2025-01-07
