Robo4 deficient mice are more susceptible to HSV-1 infection.

R4 KO mice were infected with HSV-1 RE. (A) SK lesion and angiogenesis severity were significantly increased in R4 KO mice compared to WT mice on day 15 pi. (B) Representative eye photos show increased SK lesion and angiogenesis severity in R4 KO compared to WT mice (C) Eyes were processed for cryo-sections on day 15 pi. Hematoxilyn and eosin staining was carried out on 6 μm sections, and pictures were taken at 40x magnification. The sections show decreased cellular infiltration in R4 KO mice compared to WT mice. Data are representative of three independent experiments and show mean values ± SEM (n = 15 mice/group). ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05. Statistical levels of significance were analyzed by t test.