Expression profiling in STAG2 mutant K562 cells

K562 (female chronic myelogenous leukemia) cells were CRISPR-Cas9 edited to contain STAG2 R614* mutation. STAG2 is encoded on the X-chromosome and normally mutation on one allele is sufficient for complete loss of STAG2 protein due to the wild type allele being mosaically silenced. However, we obtained both heterozygous and homozygous mutant lines that showed partial and complete loss of STAG2 protein respectively. We here examined the impact of STAG2 R614* mutation on gene expression and chromatin acessibilty. RNA-sequencing, in triplicate, on the parental or wild type K562 cells (K562WT), two K562 cell lines that are heterozygous (STAG2heterozygous) for STAG2 R614* mutation and two K562 cell lines that are homozygous for STAG2 R614* (STAG2homozygous) mutation.