Loss of the starvation-and-light fruitbody formation trigger in the myxomycete Physarum roseum

Myxomycetes are unicellular amoebozoans that form fruiting bodies to reproduce, a process known as sporulation. In the model species Physarum polycephalum, plasmodia form fruiting bodies only after several days of starvation followed by light exposure. It has long been assumed that the same starvation-plus-light trigger applies to the genus Physarum. Recent observations of congeners that fail to sporulate under the same conditions have raised doubts about this assumption and prompted tentative taxonomic reconsideration. Because comparable starvation　and light tests are rare for other species of Physarum, their phenotypes and molecular mechanisms remain unclear.Consequently, we investigated Physarum rigidum and Physarum roseum under starvation and light conditions. Four of the six *P. rigidum *plasmodia sporulated by day 6, whereas P. roseum did not sporulate within seven days. RNA‑seq of P. roseum across nutrient‑rich/starved and dark/light condit..., , # Loss of the starvation-and-light fruitbody formation trigger in the myxomycete Physarum roseum

Dataset DOI: [10.5061/dryad.hmgqnk9wh](10.5061/dryad.hmgqnk9wh)

## Description of the data and file structure

Spectrum of light under “light conditions” in the experimental environment. The photon flux density (PFD) under the sporulation-inducing conditions (indirect sunlight through a translucent panel with ambient LED room lighting) was measured using a Nippon Medical & Chemical Instruments Co., Ltd. Light Analyzer LA-105. The measurements were performed by placing the instrument at the exact location where plasmodium was positioned for sporulation induction. PFD values correspond to the wavelength range 380–780 nm.

### Files and variables

#### File: ESM_2_light_condition.pdf

**Description:** Supplementary figure S1. Spectrum of light under “light conditions” in the experimental environment.

#### File: ESM2.jpeg

**Description:** Original image data for the spectrum of light under “...,