Survival, calpox viral load in blood and tissues and seroconversion after intravenous and intranasal inoculation of different doses of calpox virus.

acalculation of the copy numbers for both, calpox virus and c-myc is based on the mean value of duplicate measurements and a respective quantified plasmid standard for each real-time PCR assay.
bseroconversion, detection of IgM antibodies in plasma by immunofluorescence assay at the time point of death or at pre-determined end of experiment (surviving animals).
cviral load was expressed as calpox GE/106 c-myc genomic DNA; all tissues that were analyzed are indicated, from infected animals all tissues tested were positive. Viral load was defined as low = equal or below 105GE, or high = equal or above 107GE. Tissue abbreviations: sk: skin; he: heart; lu: lung; li: liver, sp: spleen, ki: kidney; ln: lymph node (axillaris, mesenterialis, submandibularis); bl: bladder; st: stomach; es: esophagus; si: small intestine; co: colon; br: brain; to: tonsils; tr: trachea.
dpre-determined endpoint of experiment for animals without sign of infection.
eanimals not infected during previous experiment(s) were re-infected.
fanimal was sacrificed at end of experiment.
i.v.: intravenous, i.n.: intranasal; na: no sample available; nd: not detectable; the detection limit for both assays was 10 copies; GE: genome equivalents (genomic DNA).