Expression data from Insulin Degrading Enzyme-Knocked Out hepatome human cells

Insulin-degrading enzyme (IDE) is a protein with proteolytic and non-proteolytic functions that regulates glucose homeostasis.We hypothesize that IDE deficiency alters glucagon signaling and thereby gluconeogenesis. To test this hypothesis we performed microarray studies in HepG2 cells with a complete deficiency of IDE gene. Results show that IDE deficiency led to upregulation of genes involved in cellular functions related to membranes, organelles and signaling receptors. We used microarrays to find genes whose expression is affected by the absence of IDE gene expression in hetapome human cells We used control human hepatoma cells (HepG2) and HepG2-IDE-KO cells generated using the CRISPR/Cas9 tool. Microarray analyses were performed on the ClariomTM S Array (Thermofisher). Data were acquired on the GeneChipTM 3000 instrument (Affymetrix) and high-throughput automated processing was performed using the GeneTitan™ Microarray System (Thermofisher).