Impact of Amplification Efficiency on TL Measurement via qPCR

Comparison of methods to calculate telomere length (TL). In this project we compared how amplification efficiency estimates derived from the Rotor-Gene Q rotary thermocylcer instrument compared to those generated using LinRegPCR. Furthermore, we compared how resulting T/S ratios calculated using these different approaches compared in their performance with external validity metrics.<br>Replicate level dataset hosts Ct values, T and S estimates, and related metrics of these values such as standard deviation and coefficient of variation across replicate reactions. <br>Sample level dataset contains T/S ratios generated from summarized results in replicate level dataset. Each row contains the T/S ratio of a given individual at a given time point. Some rows also contain paired T/S ratios from blood and buccal samples that were collected at the same time point for cross tissue comparisons. <br>Plate level dataset contains efficiency estimates for the various approaches utilized in the manuscript text. These include efficiencies estimated by the RotorGene Q standard curve, LinRegPCR log-linear reconstruction, and LinRegPCR standard curve. <br>Matched dataset contains T/S ratios of individuals nested by family ID for up to three generations within a family. Each row contains a parent-offspring pair. In some cases a single parent is represented on more than one row as some parents had multiple offspring. <br>Together these datasets comprise all the data used to generate the findings reported in the publication linked below.