Gene expression in prostate cancer cells chronically exposed or not to pollutant Aldrin. Gene expression in prostate cancer cells chronically exposed or not to pollutant Aldrin

DU145 prostate cancer cells were exposed for 50 days to a very low and no-toxic concentration of Aldrin (1 mM). We used microarrays to detect potential effects of Aldrin in gene expression under chronic exposure to Aldrin. Overall design: DU145 cells were seeded in a 6-well plate at density of 2x10e5 per well in the presence of 1uM of Aldrin to a final volume of 2ml of supplemented RPMI (final concentration of DMSO 0.01%). Cells were cultured in standard conditions and diluted every 3 days. Cultures were treated after every passage to complete the 50-day treatment.