ExtFig5E_qPCR_R3_LG303.xlsx
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HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, STOML2, YKT6, and TUBA1B genes, either the wildtype sequences or sequences with the GCUG mutated, as well as either empty vector or PR8 PA-X. RNA was extracted and levels were quantified by qRT-PCR
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Gaglia, Marta创建时间:
2023-01-19



