CRISPR screening of oxaliplatin in SW480

For this screen, we chose the single plasmid system of Brunello library, where each plasmid backbone contains SpCas9 genetic information and a unique sgRNA. The constructed Brunello lentiviral library was transduced into the CRC cell line SW480 by transducing the lentiviral library at a low multiplicity of infection. After 24 h of infection, we used a medium containing puromycin to select the transduced cells for 7 days to obtain mutant cells pool. A certain number of mutant cells were collected as the Baseline group and then treated with vector or oxaliplatin for 10 d, respectively.