RNA-seq of chondrogenesis following siRNA depletion of SERPINA3

SERPINA3 knockdown was observed to reduce cartilage pellet size following differentiation of mesenchymal stem cells (MSCs) to chondrocytes. To understand the global effects on the chondrocyte transcriptome, RNA-sequencing was conducted on primary MSCs depleted of SERPINA3 with siRNA and compared to cells transfected with a siRNA control over a 7 day period. RNA sequencing following mesenchymal stem cells throughout chondrogenesis at three different time points with 3 donors as biological replicates with or without SERPINA3 depletion.