Gene expression profiling of the palate in mice with a conditional inactivation of Tgfbr2 at E14.5

We performed global gene expression analyses of the palatal tissue of Tgfbr2fl/fl;Wnt1-Cre and Tgfbr2fl/fl control mice at embryonic day E14.5. Total RNA samples (1 mcirogram per sample) were converted into biotin-labeled cDNA using the EnzoTMBioArray Terminal Labeling Kit with Biotin-ddUTP and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cDNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39,000 transcripts. Microarrays were hybridized, processed and scanned, using manufacturers' recommended conditions. Each of the .cel files represents a different experiment (biological replicate). E145_CKO files are results from day 14.5 Tgfbr2fl/fl;Wnt1-Cre mutant mice. E145_WT files are results from day 14.5 Tgfbr2fl/fl control mice.