Substrate identification by PamChip® 144 peptide microarray.

Peptide ID is based on the UniProt Knowledgebase, and the numbers indicate the position of the first and last amino acids of the peptide in the complete human protein (UniProt annotation and numbering). Substrates were defined as those peptides showing protein- and ATP-concentration dependent signals after incubation. Nonspecific signals were not considered. In the three columns on the right hand side of the table, an X indicates phosphorylation of that peptide by the JAK2 construct indicated in the heading.