Data from: Comparative analysis of genome sequences from four strains of the Buchnera aphidicola Mp endosymbiont of the green peach aphid, Myzus persicae

Myzus persicae, the green peach aphid, is a polyphagous herbivore that feeds on hundreds of species of mostly dicot crop plants. Like other phloem-feeding aphids, M. persicae rely on the endosymbiotic bacterium, Buchnera aphidicola (Buchnera Mp), for biosynthesis of essential amino acids and other nutrients that are not sufficiently abundant in their phloem sap diet. Tobacco-specialized M. persicae are typically red and somewhat distinct from other lineages of this species. To determine whether the endosymbiotic bacteria of M. persicae could play a role in tobacco adaptation, we sequenced the Buchnera Mp genomes from two tobacco-adapted and two non-tobacco M. persicae lineages.
With a genome size of 643.5 kb and 579 predicted genes, Buchnera Mp is the largest Buchnera genome sequenced to date. No differences in gene content were found between the four sequenced Buchnera Mp strains. Compared to Buchnera APS from the well-studied pea aphid, Acyrthosiphon pisum, Buchnera Mp has 21 additional genes. These include genes encoding five enzymes required for biosynthesis of the modified nucleoside queosine, the heme pathway enzyme uroporphyrinogen III synthase, and asparaginase. Asparaginase, which is also encoded by the genome of the aphid host, may allow Buchnera Mp to synthesize essential amino acids from asparagine, a relatively abundant phloem amino acid.
After validation, a final genome sequence was constructed for each Buchnera Mp chromosome and plasmid. These sequences have been deposited in Genbank. Supplemental tables provide data on (S1) Collection information and microsatellite genotype of Myzus persicae lineages hosting the four sequenced Buchnera Mp strains; (S2) Twenty-one Buchnera Mp coding sequences whose counterparts are absent in Buchnera APS; (S3) Thirteen nonsynonymous mutations in the Buchnera Mp genomes of lineages W106, F009 and G002 that are predicted to be deleterious.
Together results indicate that the obligate intracellular symbiont Buchnera aphidicola does not contribute to the adaptation of Myzus persicae to feeding on tobacco. Resources in this dataset:Resource Title: Comparative analysis of genome sequences from four strains of the Buchnera aphidicola Mp endosymbion of the green peach aphid, Myzus persicae. File Name: Web Page, url: https://doi.org/10.1186/1471-2164-14-917 Data from the paper is linked in related content of this record.

桃蚜（Myzus persicae），又称绿色桃蚜，是一种多食性植食性昆虫，以数百种主要为双子叶作物植物为食。与其他韧皮部食性蚜虫类似，桃蚜依赖共生细菌 Buchnera aphidicola（Buchnera Mp）的生物合成作用，以获取其韧皮部汁液饮食中含量不足的必需氨基酸和其他营养素。烟草专化的桃蚜通常呈红色，与其他种群的谱系有所区别。为了确定桃蚜的共生细菌是否在烟草适应性中发挥作用，我们对来自两种烟草适应型和两种非烟草桃蚜谱系的 Buchnera Mp 基因组进行了测序。Buchnera Mp 的基因组大小为 643.5 kb，预测有 579 个基因，是目前测序的最大 Buchnera 基因组。四个测序的 Buchnera Mp 稀释剂之间在基因内容上没有发现差异。与被广泛研究的豌豆蚜虫 Acyrthosiphon pisum 中的 Buchnera APS 相比，Buchnera Mp 有 21 个额外的基因，包括编码五个酶的基因，这些酶是合成修饰核苷酸 queosine 的必需，还有血红素途径酶 uroporphyrinogen III 合成酶和天冬酰胺酶。天冬酰胺酶也被蚜虫宿主的基因组编码，可能允许 Buchnera Mp 从相对丰富的韧皮部氨基酸天冬酰胺中合成必需氨基酸。经过验证，为每个 Buchnera Mp 染色体和质粒构建了最终的基因组序列。这些序列已存入 Genbank。补充表格提供了有关（S1）携带四个测序 Buchnera Mp 稀释剂的桃蚜谱系的收集信息和微卫星基因型；（S2）21 个 Buchnera Mp 编码序列，这些序列在 Buchnera APS 中不存在；（S3）在谱系 W106、F009 和 G002 的 Buchnera Mp 基因组中预测为有害的 13 个非同义突变的数据。综合结果表明，义务性细胞内共生菌 Buchnera aphidicola 并不影响桃蚜对烟草的适应性。本数据集中的资源包括：资源标题：《绿色桃蚜 Buchnera aphidicola Mp 内共生菌的四株基因组序列的比较分析》。文件名：网页，网址：https://doi.org/10.1186/1471-2164-14-917。论文中的数据链接在本记录的相关内容中。